| 1. | Determination of sulfur amino acids in feedstuffs - ion exchange chromatography
饲料中含硫氨基酸测定方法-离子交换色谱法 |
| 2. | Sodium tripolyphosphate for industrial use - separation by ion exchange chromatography and determination of the different phosphate forms
工业三聚磷酸钠离子交换柱色谱法分离测定不同形式的磷酸盐 |
| 3. | A simple , sensitive , accurate and rapid determination method for chloride by suppressed ion exchange chromatography was developed
本文研究了用离子色谱测定玉米粉中氯含量方法，方法与传统的化学分析方法相比具有灵敏、简便、快速、准确特点。 |
| 4. | The purified badh , which became a single band in sds - page , has been obtained by a combination of ammonium sulfate fractionation , ion exchange chromatography and gel filtration chromatography
辽宁碱蓬甜菜碱醛脱氢酶分子量为129kd ，单个亚基的分子量为64 . 5kd ，表明其为同型二聚体。 |
| 5. | But the whole level of serum titer in combined vaccine group was higher than others . igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose
对分离到的血清用饱和硫酸铵盐析法提取igg ，并用deae纤维素离子交换层析法对提取的igg进行纯化。 |
| 6. | Methods 1 mr1 hybridoma was cultured in hollow fibre system . culture supernatant was harvested , then precipitated by half - saturated ammonia sulphate . mfm was purified by deae - sephacel ion exchange chromatography . mri concentration was measured by elisa
硫酸钱半饱和沉淀， oeae阴离子交换层析分离纯化抗co40l单克隆抗体mri 。 pbs透析后， 505一队ge检测纯化抗体的纯度， eusa测定抗体浓度。 |
| 7. | Although ion exchange chromatography is used popularly in determination of chloride in environmental , biological samples , it is rarely used in the determination of chloride in plant because it is easily affected by the ph and the matter coexisted
摘要离子色谱分析虽已广泛应用于食品、农业、环境保护及医疗卫生等日常监测和研究工作，但由于其对待测组份的共存干扰物质及酸碱度要求较苛刻，因此目前用于谷物类样品分析的方法还较少。 |
| 8. | One is a combination of ion exchange chromatography on q sepharose ff , hydrophobic interaction chromatography on phenyl hp , gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q . the other is a combination of ion exchange chromatography on q sepharose ff , two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially
上清液中重组蝗蛇毒类激血酶的纯化是通过设计的不同分离纯化方法组合进行的，并对每一种组合进行了活力与纯度及口收率的评价，结果表明两种组合方式都具有一定的实用性和可操作性。 |
| 9. | Fusion expression of m - centrin in e . coil bl21 was performed by induction of fptg . fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography . the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌体经过超声波裂解后得到的上清夜经过gst亲和层析后用prescissionprotease ( ppase )酶切，酶切产物再次经过gst亲和层析和hitrapq阴离子交换层析两步柱层析纯化后，得到纯度较高的的m - centrin 。 |
| 10. | Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。 |
